Recent Advances in Chiral Analysis of Proteins and Peptides

Like many biological compounds, proteins are found primarily in their homochiral form. However, homochirality is not guaranteed throughout life. Determining chiral proteinogenic sequence a complex analytical challenge. This because certain d-amino acids contained play role human health and disease. the case, for example, with d-Asp elastin, ?-amyloid ?-crystallin which, respectively, have an action on arteriosclerosis, Alzheimer’s disease cataracts. Sequence-dependent sequence-independent two strategies detecting presence position of proteins. These methods rely enzymatic digestion by site-specific enzyme acid hydrolysis deuterium or tritium environment to limit natural racemization amino acids. In this review, chromatographic electrophoretic techniques, such as LC, SFC, GC CE, will be recently developed (2018–2020) enantioseparation peptides. For future work, discovery development new stationary phases derivatization reagents could increase resolution separations.